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KMID : 0355420100340010001
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Journal of Korean Academy of Oral Health
2010 Volume.34 No. 1 p.1 ~ p.8
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The effect of xylitol on the use of various carbohydrates by Streptococcus mutans
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Kim Ji-Hye
Lee Young-Eun
Chung Sun-Young
Ahn Sang-Hun
Choi Youn-Hee
Song Keun-Bae
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Abstract
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Objective: Xylitol prevents caries by inhibiting the growth and virulence of Streptococcus mutans. However, most reports only examined the presence of specific carbohydrate such as glucose or sucrose. We therefore tested the effect of xylitol on S. mutans in several sugars.
Methods: Basal culture medium for S. mutans KCTC3065 was TYE medium containing 0.5% glucose (G-TYE). The strain was cultured in 10 ml basal culture medium adding 0.5% carbohydrates (sucrose, fructose, maltose) with or without 1% xylitol at 37℃ under 10% CO2 for 24 h. Cells were collected for analysis growth, acid production, synthesis of extracellular polysaccharides, and gene expression.
Results: Xylitol reduced the growth rate of S. mutans in the presence of G-TYE and maltose (p＜0.05), as well as the final growth of S. mutans in the presence of G-TYE, sucrose, and maltose (p＜0.05). Xylitol inhibited acid production in the presence of G-TYE and maltose (p＜0.05), but not in sucrose and fructose (p＞0.05). Xylitol reduced the synthesis of extracellular polysaccharides and the expression of gtfB and gtfD in all carbohydrates.
Conclusions: Xylitol inhibited growth and acid production of S. mutans in the presence of G-TYE and maltose, and decreased extracellular polysarccharides synthesis in all carbohydrates.
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KEYWORD
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Streptococcus mutans, carbohydrates, xylitol
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